Fig. 1. Cisplatin (CP) induces mouse renal proximal tubular epithelial cell (mTEC) apoptosis and H3 lysine 27 trimethylation (H3K27me3) in a dose-dependent manner.

mTECs were exposed to various concentrations of cisplatin for 24 h. Cell viability was detected by cell counting kit-8 (CCK-8) assay (a). Cell lysates were prepared and subjected to immunoblot analysis with antibodies against caspase3 (cas3) and GAPDH (b), enhancer of zeste homolog 2 (EZH2), H3K27me3, GAPDH, H3, and tubulin (e). The levels of cleaved-cas3 (c), EZH2 (d), or H3K27me3 (f) were quantified by densitometry and normalized with GAPDH or histone H3 as indicated. Data represent the mean ± SEM of at least three experiments. Bars with different letters (a–f) for each molecule are significantly different from one another (P < 0.05)