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. 2019 May 1;10(5):355. doi: 10.1038/s41419-019-1589-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — mTECs were treated with 20 μg/ml of cisplatin for the indicated time. Cell lysates were prepared and subjected to immunoblot analysis with antibodies against Cleaved caspase 3(Cleaved-Cas3), EZH2, H3K27me3, and β-actin (a). The levels of cleaved-cas3 (b), EZH2 (c), or H3K27me3 (d) were quantified by densitometry and normalized with β-actin. Data represent the mean ± SEM of at least three experiments. Bars with different letters (a–c) for each molecule are significantly different from one another (P < 0.05)