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. 2019 May 1;10(5):355. doi: 10.1038/s41419-019-1589-y

Fig. 5. 3-DZNeP inhibits CP-induced mTEC apoptosis by a mitogen-activated protein kinase (MAPK) independent restoration of E-cadherin.

Fig. 5

mTECs were treated with 20 μg/ml of cisplatin for 0–24 h (a–e) or pretreated with 3-DZNeP (0–20 μM) and then exposed to cisplatin (20 μg/ml) for an additional 24 h (f–i). Cell lysates were prepared and subjected to immunoblot analysis with antibodies against JNK phosphorylation (p-JNK), ERK phosphorylation (p-ERK), p38 phosphorylation (p-p38), E-cadherin, and β-actin (a, f, h). The levels of p-JNK (e, g), p-ERK (d, g), p-p38(c, g), or E-cadherin (b, i) were quantified by densitometry and normalized with β-actin. Data represent the mean ± SEM of at least three experiments. Bars with different letters (a–f) for each molecule are significantly different from one another (P < 0.05)