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. 2019 May 1;9:6748. doi: 10.1038/s41598-019-42952-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Detection of elastase and protease LasA in the P. aeruginosa cell-free secretome. Cell-free supernatants obtained from 24 h cultures of PAO1 and CF isolates 5, 6 and 7 grown under normoxia (+) and anoxia (−) were subject to protein precipitation, SDS-PAGE electrophoresis and MS analysis. Data represents N = 1 from five pooled samples. The abundance of elastase and protease LasA is expressed as fold-change compared to normoxia.