Figure 5.

Heat-treatment and size fractionation of P. aeruginosa cell-free supernatants upon the growth of S. aureus. Cell-free supernatants from P. aeruginosa grown under normoxia or anoxia were added to standardised S. aureus directly or were first subjected to 3 kDa size fractionation, with select fractions then being subjected to boiling. Plates were incubated at 37 °C for 14 h and the OD₄₇₀ of S. aureus cultures read at hourly intervals. Plots represent the mean OD at 14 h ± S.D. of two individual experiments (N = 2), each performed in duplicate. WS: Whole supernatant, WSHI: Whole supernatant heat-inactivated, >3: greater than 3 kDa, >3HI: greater than 3 kDa heat-inactivated, <3: less than 3 kDa, <3HI: less than 3 kDa heat-inactivated. Statistical differences were determined using one-way ANOVA with Dunnett’s post-hoc (versus S. aureus only control). *P < 0.05, **P < 0.01 ***P < 0.001.