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. 2019 May 1;9:6748. doi: 10.1038/s41598-019-42952-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Heat-treatment and size fractionation of S. aureus cell-free supernatants upon P. aeruginosa swimming and swarming motility. Swimming and swarming motilities of the select P. aeruginosa isolates were assessed following an overnight incubation on swimming and swarming agar plates. S. aureus only motility values were taken from Fig. 6. WS: whole supernatant fractions of S. aureus culture supernatant were taken from Fig. 6. Plates were supplemented with a 1:100 dilution of S. aureus culture supernatant fractions (>3 or <3 kDa), with select fractions also being subject to heat-treatment. >3: greater than 3 kDa, >3HI: greater than 3 kDa heat-inactivated, <3:less than 3 kDa, <3HI: less than 3 kDa heat-inactivated. Data are presented as mean ± S.D from two independent experiments (N = 2) each performed in duplicate. Statistical differences were determined using one-way ANOVA with Dunnett’s post-hoc (versus WS: whole supernatant only).