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. 2019 May 1;9:6788. doi: 10.1038/s41598-019-43210-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of IFN-γ (A) and IL-17A (B), measured by RT-PCR, in pooled spleen and axillary lymph nodes of vaccinated Myotis lucifugus after challenge with Pseudogymnoascus destructans (Pd) and ex vivo recall with calnexin or Pd. Bats had been vaccinated with raccoon poxviruses (RCN) expressing calnexin and serine protease (RCN-CAL/RCN-SP), via intramuscular (IM; n = 8) or oral (n = 7) administration, or sham vaccinated with RCN expressing luciferase (RCN-luc; n = 3). Bars and error bars represent average n-fold changes (indicated by the number above the error bar) and standard errors, normalized to the non-stimulated (medium) control. Values marked with an asterisk are significantly different from the medium control at P < 0.05.