Fig. 3.

DNA methylation status of TSDRs in WT and Tet2/3^fl/flFoxp3^Cre DKO Treg cells. a–f DNA methylation status of TSDRs (Treg-specific demethylated regions). Graphs depict the percentage of 5mC + 5hmC determined by BS-seq in peripheral Tregs in: (a) 4 CpGs in Foxp3 CNS1, and (b) 11 CpGs in Foxp3 CNS2, (c) 4 CpGs in Il2ra intron 1a, (d) 7 CpGs in Tnfrsf18 exon 5, (e) 6 CpGs in Ikzf4 intron 1b and (f) 5 CpGs in Ctla4 exon 2. Error bars show mean ± s.d. of thousands of sequencing reads from two independent experiments. Wild type (WT) Treg cells (8–10 weeks-old) are shown in black, CD4^Cre mediated Tet2/3 DKO Treg cells (3–4 weeks-old) are shown in red¹² and Foxp3^Cre mediated Tet2/3 DKO Treg cells (8–10 weeks-old) are shown in blue