Fig. 1. SIRT1 expression is increased to promote chemoresistance.

a SIRT1 expression in H460-R and H460 lung cancer cells were determined by Western blotting. b Western blot analysis of SIRT1 expression in H460 cells transfected with lentivirus carrying empty vector or SIRT1-expressing vector. c The viability of H460 cells with SIRT1 overexpression after treatment with cisplatin was indicated were measured by MTS assay. d Analysis of apoptosis by Annexin V/propidium Iodide staining with Flow cytometry. Data represents in triplicate and shown as the mean ± S.D. *p < 0.05. e Protein level of cleaved-PARP1, cleaved-Caspase3 and Ɣ-H2AX in H460 cells after cisplatin treatment were analyzed by Western blotting