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. 2019 May 1;10(5):363. doi: 10.1038/s41419-019-1592-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Interaction of SIRT1 with XRCC1 in H460-R cells was determined by co-immunoprecipitation (Co-IP). b Effect of SIRT1 siRNAs on acetylation of XRCC1 in H460-R cells was determined by anti- XRCC1 antibody immunoblotting after immunoprecipitation with anti-Pan-acetyl lysine antibody. c Effect of SIRT1 inhibitors on acetylation of XRCC1 in H460-R cells was determined as in (b). d The acetylation of XRCC1 in NAM-treated H460-R cells was analyzed by mass spectrometry (MS). The MS spectrum of peptide XRCC1 showed that lysine 237, 260, 298, 431 was acetylated in NAM-treated XRCC1. e Alignment of XRCC1 acetylated sites in various species. f Acetylation of wild type or mutated XRCC1 in HEK293 cells was determined by anti- Pan-acetyl lysine antibody immunoblotting after immunoprecipitation with anti-flag antibody. g Effect of EX-527 on the expression of wild type or mutated XRCC1 in HEK293 cells was determined by anti-flag immunoblotting. h Effect of EX-527 on half-life of wild-type or mutated XRCC1 in CHX-treated HEK293 cells was analyzed by immunoblotting with anti-flag antibody. i Interaction of wild type or mutated XRCC1 with β-TrCP in HEK293 cells before and after treatment with EX-527 were analyzed by co-immunoprecipitation with anti-Flag antibody and immunoblotting with anti- β-TrCP antibody