Figure 3.

Ab responses induced by eGn and mCherry encoding plasmids. (A) Sera from pscDEC205-eGn, pscCtlr-eGn, and peGn (± pGM-CSF) immunized Balb/c (7 per group) were collected at day 0, 21, 42, 60, and were assayed for the detection of eGn-specific IgG using lysate from HEK293 cells transfected with peGn as coating. The OD signals at 450 nm from the individual sera collected at each time point and tested at a 1:100 dilution are shown. Each animal is represented with a distinct symbol and the mean OD value is indicated. P-values were determined using to the Mann-Whitney test (*p < 0.05; **p < 0.01). The significant difference between the OD values measured at each time point in the vaccinated vs. the PBS group is indicated by a box for clarity (p < 0.05). This experiment has been repeated twice with similar results. (B) Sera from pscDEC205-mCherry, pscCtlr-mCherry (± pGM-CSF) immunized Balb/c (7 per group) were collected at day 0, 21, 42, 60, and mCherry-specific IgG were evaluated in 1:100 diluted sera using recombinant mCherry as a coating antigen. Each animal is represented with a distinct symbol and the mean is indicated (n = 6–7 per group). The experiment has been repeated twice with similar results. P-values were determined using to the Mann–Whitney test (*p < 0.05; **p < 0.01). The significant differences between the OD values measured at day 0 in comparison to days 21, 42, and 60 are indicated by a box for clarity and were calculated using a non-parametric paired Wilcoxon test (p < 0.01).