Table 1.
Protection mediated by the T cells or sera from peGn-immunized mice upon RVFV challenge.
| Liver | Brain | ||||||
|---|---|---|---|---|---|---|---|
| Group | n° of mice surviving/total n° of mice | DPCa | % of initial weightb | Viral RNA (RNA copy/g) | Viral titer (pfu/g) | Viral RNA (RNA copy/g) | Viral titer (pfu/g) |
| Control mice (PBS + pGM-CSF) | 2/5 | 9I | 78.4 | ND | ND | ND | ND |
| 12I | 66.6 | 1,13E+06 | – | 2,28E+09 | 3,67E+06 | ||
| 12I | 86.4 | 1,37E+06 | – | 2,05E+09 | 2,60E+07 | ||
| 22 | 99.1 | – | – | – | − | ||
| 22 | 95.4 | – | – | – | − | ||
| Immunized mice (peGn + pGM-CSF) | 5/5 | 22 | 101.7 | – | – | – | − |
| 22 | 98.3 | – | – | – | − | ||
| 22 | 99.5 | – | – | – | − | ||
| 22 | 100.4 | – | – | – | − | ||
| 22 | 102.8 | – | – | – | − | ||
| Transfer control T cells (PBS + pGM-CSF) | 2/5 | 5I | 96.2 | ND | ND | ND | ND |
| 8I | 70.1 | 6,21E+06 | 4,40E+02 | 7,72E+10 | 5,65E+07 | ||
| 11I | 64.1 | 2,98E+06 | – | 3,03E+10 | 1,94E+08 | ||
| 22 | 105 | – | – | 2,13E+08 | − | ||
| 22 | 102.6 | – | – | 4,71E+09 | − | ||
| Transfer immunized T cells (peGn + pGM-CSF) | 1/5 | 4I | 90.6 | ND | ND | ND | ND |
| 8I | 69.2 | 1,36E+07 | – | 2,75E+13 | 3,84E+09 | ||
| 8I | 76.7 | 8,70E+06 | – | 9,58E+09 | 5,78E+07 | ||
| 9I | 81.5 | – | – | 8,31E+11 | 7,16E+08 | ||
| 22 | 95.1 | – | – | 1,32E+09 | − | ||
| Transfer control serum (PBS + pGM-CSF) | 0/7 | 4I | 91.2 | 6,50E+10 | 1,33E+06 | 1,98E+10 | − |
| 6I | 88.6 | ND | ND | ND | ND | ||
| 8I | 73.4 | ND | ND | ND | ND | ||
| 8I | 89.3 | ND | ND | ND | ND | ||
| 8I | 71.5 | 1,20E+07 | 1,11E+04 | 5,57E+12 | 1,25E+09 | ||
| 8I | 74.2 | 1,18E+07 | – | 4,86E+12 | 8,24E+08 | ||
| 9I | 72.2 | 1,45E+07 | – | 4,94E+10 | 3,27E+08 | ||
| Transfer immunized serum (peGn + pGM-CSF) | 5/7 | 8I | 78.8 | ND | ND | ND | ND |
| 10I | 82.3 | 1,70E+06 | – | 9,02E+09 | 4,87E+07 | ||
| 22 | 107.7 | – | – | – | − | ||
| 22 | 101.5 | – | – | 2,39E+09 | − | ||
| 22 | 107.3 | – | – | – | − | ||
| 22 | 107.5 | – | – | 1,26E+09 | − | ||
| 22 | 108.8 | – | – | 1,50E+09 | − | ||
Mice were immunized, transferred, and challenged as described in Figure 1.
a
Death, days post challenge (DPC) either due to disease (I) or euthanized during the challenge protocol;
b
Values on the day before death; –, non-detected; ND, not done.