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. 2018 Dec 26;28(10):1594–1607. doi: 10.1093/hmg/ddy448

Figure 1.

Figure 1

GRP75 interacts with frataxin both in vivo and in vitro. GRP75 but not control IgG immunoprecipitated frataxin and ISCU2 from both cortical homogenates (A) and neuronal lysates (B). Purified human full-length frataxin (frataxin1–210-6XHis) (precursor) also pulled down GRP75 from cortical homogenates while control experiments with frataxin1–210-6XHis omitted (beads only) showed no immunoreactivity. NFS1 and HSP60 served as a positive and negative controls, respectively (C). Similarly, purified GST-GRP75 but not GST pulled down both frataxin1–210-6XHis (D) and mature frataxin (frataxin81–210-6XHis) (E) in an in vitro binding assay. GST-GRP75 bound strongly to frataxin precursor when compared with mature frataxin in an in vitro binding assay (F).