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. 2019 Apr 9;16:481–493. doi: 10.1016/j.omtn.2019.03.009

Figure 2.

Figure 2

Effect of miR-744 Overexpression on the Cell Proliferation

(A–G) Myoblasts treated with pcDNA3.1 or pcDNA3.1-miR-744 were subsequently incubated with growth medium for 24 h and cell phase analyzed (A) and counted (B) by flow cytometry. (C) Cell proliferation index was measured by cell counting kit-8 (CCK-8) assay. (D) Cell proliferation was measured by MTT assay. (E) Cell proliferation was measured with 5-ethynyl-20-deoxyuridine (EdU); the scale bars represent 200 μm. (F and G) The expression of proliferation marker gene PCNA, CDK2, and cyclin D1 was measured by real-time qPCR (F) and western blotting (G). Values are mean ± SEM for three biological replicates; *p < 0.05; **p < 0.01.