Figure 4.

Effects of Wnt5a Overexpression and CaMKIIδ Silencing on the Cell Proliferation and Differentiation

(A and B) Bovine primary myocytes were treated with pcDNA3.1-Wnt5a (A) or si-CaMKIIδ (B), and the mRNA and protein levels of proliferation marker genes were measured by real-time qPCR and western blotting. (C and D) Bovine primary myocytes were treated with pcDNA3.1-Wnt5a (C) or si-CaMKIIδ (D), and cell proliferation was measured with 5-ethynyl-20-deoxyuridine (EdU); the scale bars represent 200 μm. (E and F) Bovine primary myocytes were treated with pcDNA3.1-Wnt5a (E) or si-CaMKIIδ (F), and the mRNA and protein expression of differentiation marker genes MyoG, MyoD, and MyHC was measured by real-time qPCR and western blotting. (G and H) C2C12 cells were treated with pcDNA3.1-Wnt5a (G) or si-CaMKIIδ (H), and cell differentiation was measured by immunofluorescence; the scale bar represents 200 μm. Values are mean ± SEM for three biological replicates; *p < 0.05; **p < 0.01.