Figure 7.

Overexpression of circSNX29 Promotes Activation of Wnt5a/Ca²⁺ Pathway by Regulating miR-744-Wnt5a/CaMKIIδ Axis

(A) KEGG enrichment pathways result. (B and C) The downstream mediators of Wnt5a were detected by qPCR (B) and western blotting (C). (D) Schematic presentation of Wnt5a/Ca²⁺/calcineurin/NFATC1 signaling pathways. (E) Analysis of the intracellular variation of calcium concentration by confocal microscopy. (F) CamKII activity assays. (G) Western blotting on myoblasts to detect the levels of phosphorylated PLC and PKC. (H and I) The expression of calcineurin and NFATC1 was detected by qRT-PCR (H) and western blotting (I). Values are mean ± SEM for three biological replicates; *p < 0.05; **p < 0.01.