Figure 4.
PER levels were reduced in pacemaker neurons of AMPK subunit knockdown flies. A, B, C, D, Brains from flies of the indicated genotypes were dissected at ZT2 and stained with anti-PER (Rb1, green) and anti-PDF (C7, red) antibodies. The dorsally projecting neurites from sLNvs (arrowheads) showed morphological defects (B) or were missing (D). E, F, G, PER fluorescence intensities in lLNv, LNd, and DN1 neurons were quantified. Values indicate mean ± SEM (n = 11–35). One-way ANOVA with Tukey's test: *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001).