Figure 6.

The effect of a dynamin inhibitor on RRP exocytosis. A, B, We tested the effect of 30 μm Dyngo-4a (dynamin inhibitor) on the ΔC_m response in P15–P21 IHCs from WT (A) and in Otof^−/− mice (B) by applying five consecutive brief 20 ms depolarizing pulses. C, The presence of Dyngo-4a increased the net ΔC_m responses in WT IHCs by blocking endocytosis. *p < 0.05 (unpaired t test). Dyngo-4a did not affect the ΔC_m responses in Otof^−/− IHCs. D, The increase in ΔC_m responses in WT IHCs was not the result of augmented Ca²⁺ currents since similar amplitudes were found in the presence or absence of the endocytotic inhibitor. Error bars indicate SE.