Figure 2.

PRPH2 is enriched in the endosomal compartments in MDCK cells. A, Nontagged bovine PRPH2 was stably expressed in MDCK cells, and the cells were costained with anti-PRPH2 (3B6) and various transfected organelle-specific GFP reporters. Insets show enlarged views of the boxed areas. Arrowheads point to the overlapping PRPH2 and GFP signals. B, Quantification of A. Pearson's coefficients represent the extent of the colocalization between PRPH2 and the indicated organelle-specific markers (mean ± SD; n = 36 cells; N = 3). C–E, Stably expressed PRPH2-mCh (detected by mCh antibody) was colabeled with Lamp2 (C), GM130 and transfected Lamp1-GFP (D), or transfected GFP-Sec61β (E). The boxed areas were zoomed in and further acquired by super-resolution Airyscan. Arrowheads point to signals of PRPH2-mCh overlapped with either endogenous Lamp2 (C) or Lamp1-GFP (D), but not GM130. Scale bars: A, C, D, E, 10 μm.