Figure 4.

Mimicking spontaneous activity using holographic illumination. Top black trace shows cell-attached recordings of four spontaneous APs and one holography-induced AP in an example cell expressing ChrimsonR from a Cux2-CreERT2;Ai167 transgenic mouse. Middle blue trace shows the above spiking pattern precisely reproduced using five brief light pulses in the same cell. Bottom, Temporally zoomed-in raster plot of spike latencies in the original trial (black bars) and the three mimicking trials (blue bars) by aligning the light-pulse onset in the original trial with that of the second illumination in the mimicking trials. Note the submillisecond jitter of 0.78, 0.76, 0.78, 0.91, and 0.96 ms for five spikes in the four recordings.