Cross-talk activation of opsins upon 2P scanning. Top, Representative cell-attached recordings of cross-talk activation upon 920 nm laser scanning at different scanning power for a 5.92 Hz frame rate involving cells positive for ReaChR (A), CoChR (B), or ChrimsonR (C) via viral infection. Bottom, Baseline-subtracted firing rate in relation to different scan power ranges (total n = 10, 6, 5 for ReaChR, CoChR, ChrimsonR) for scanning at 5.92 Hz frame rate. Elevated cross-talk firing is more pronounced for ReaChR when scanning at a power of >60 mW. Asterisk denotes significant difference for multiple comparison between scan power ranges.