. 2019 May 1;39(18):3484–3497. doi: 10.1523/JNEUROSCI.1785-18.2018

Table 1.

Spiking properties upon 2P pulse stimulation with threshold power density

Opsin	Light-pulse duration (ms)	AP latency (ms)	AP jitter (ms)	AP count	AP probability (%)	Laser-power density^b (mW/μm²)	Estimated laser-power density^c (mW/μm²)
ReaChR	10	9.48 ± 1.41^a (n = 8)	1.84 ± 0.73^a (n = 7)	0.98 ± 0.065^a (n = 8)	90.58 ± 6.30^a (n = 8)	0.14 ± 0.018^a (n = 8)	0.072 ± 0.0083^a (n = 8)
	5	11.16 ± 2.42^a (n = 6)	1.82 ± 0.60^a (n = 6)	1.11 ± 0.070^a (n = 6)	100.00 ± 0.00^a (n = 6)	0.13 ± 0.017^a (n = 6)	0.064 ± 0.0065^a (n = 6)
	2	21.10 (n = 1)	3.05 (n = 1)	1.00 (n = 1)	100.00 (n = 1)	0.10 (n = 1)	0.054 (n = 1)
CoChR	10	8.18 ± 0.58^a (n = 7)	0.58 ± 0.13^a (n = 6)	1.00 ± 0.072^a (n = 7)	95.24 ± 4.76^a (n = 7)	0.19 ± 0.038^a (n = 7)	0.10 ± 0.018^a (n = 7)
	5	6.74 ± 0.28^a (n = 4)	1.10 ± 0.27^a (n = 4)	1.08 ± 0.083^a (n = 4)	100.00 ± 0.00^a (n = 4)	0.11 ± 0.016^a (n = 4)	0.056 ± 0.0090^a (n = 4)
	3	6.93 (n = 1)	0.36 (n = 1)	1.00 (n = 1)	100.00 (n = 1)	0.15 (n = 1)	0.058 (n = 1)
	2	4.05 (n = 1)	0.60 (n = 1)	1.00 (n = 1)	100.00 (n = 1)	0.10 (n = 1)	0.049 (n = 1)
ChrimsonR	10	8.50 ± 0.74^a (n = 10)	0.67 ± 0.23^a (n = 8)	0.89 ± 0.054^a (n = 10)	89.33 ± 5.46^a (n = 10)	0.24 ± 0.021^a (n = 10)	0.13 ± 0.015^a (n = 10)
	5	6.21 ± 0.72^a (n = 6)	1.30 ± 0.26^a (n = 6)	0.99 ± 0.082^a (n = 6)	93.33 ± 5.77^a (n = 6)	0.15 ± 0.012^a (n = 6)	0.080 ± 0.0066^a (n = 6)
	2	5.24 ± 1.06^a (n = 8)	0.30 ± 0.13^a (n = 5)	0.96 ± 0.12^a (n = 8)	87.5 ± 6.10^a (n = 8)	0.15 ± 0.021^a (n = 8)	0.073 ± 0.011^a (n = 8)
ChrimsonR Tg	15	13.32 ± 0.75^a (n = 2)	3.16 ± 1.85^a (n = 2)	0.82 ± 0.017^a (n = 2)	81.67 ± 1.67^a (n = 2)	0.38 ± 0.075^a (n = 2)	0.15 ± 0.026^a (n = 2)
	10	9.55 ± 0.66^a (n = 5)	2.39 ± 1.08^a (n = 4)	0.82 ± 0.11^a (n = 5)	77.00 ± 10.20^a (n = 5)	0.22 ± 0.012^a (n = 5)	0.11 ± 0.013^a (n = 5)
	5	5.70 (n = 1)	1.15 (n = 1)	0.75 (n = 1)	75.00 (n = 1)	0.15 (n = 1)	0.070 (n = 1)

^aMean ± SEM.

^bLaser-power density measured at the tip of objective.

^cEstimated power density by assuming the scattering length at 1030 nm as 175 μm, which is derived from simulations based on experimental data (Papagiakoumou et al., 2013).