. 2019 May 1;39(18):3484–3497. doi: 10.1523/JNEUROSCI.1785-18.2018

Table 2.

Spiking properties upon 2P pulse stimulation with submillisecond precision

Opsin	Light-pulse duration (ms)	AP latency (ms)	AP jitter (ms)	AP count	AP probability (%)	Laser-power density^b (mW/μm²)	Estimated laser-power density^c (mW/μm²)
ReaChR	10 (n = 7)	8.80 ± 0.77^a	0.58 ± 0.15^a	1.24 ± 0.19^a	100.00 ± 0.00^a	0.16 ± 0.020^a	0.081 ± 0.0092^a
ReaChR	5 (n = 4)	8.31 ± 1.68^a	0.47 ± 0.098^a	1.50 ± 0.29^a	100.00 ± 0.00^a	0.14 ± 0.021^a	0.073 ± 0.0087^a
CoChR	10 (n = 7)	7.69 ± 0.77^a	0.61 ± 0.12^a	1.05 ± 0.047^a	100.00 ± 0.00^a	0.22 ± 0.038^a	0.12 ± 0.018^a
	5 (n = 4)	5.84 ± 0.62^a	0.37 ± 0.15^a	0.92 ± 0.083^a	91.67 ± 8.33^a	0.14 ± 0.024^a	0.072 ± 0.014^a
	3 (n = 1)	6.93	0.36	1.00	100.00	0.15	0.058
	2 (n = 1)	4.05	0.60	1.00	100.00	0.10	0.049
ChrimsonR	10 (n = 8)	7.63 ± 0.55^a	0.48 ± 0.10^a	0.95 ± 0.05^a	95.00 ± 5.00^a	0.25 ± 0.033^a	0.13 ± 0.023^a
	5 (n = 5)	5.41 ± 0.87^a	0.60 ± 0.13^a	0.95 ± 0.05^a	95.00 ± 5.00^a	0.19 ± 0.033^a	0.098 ± 0.015^a
	2 (n = 8)	3.82 ± 0.24^a	0.35 ± 0.10^a	1.11 ± 0.084^a	100.00 ± 0.00^a	0.18 ± 0.013^a	0.088 ± 0.0072^a
ChrimsonR Tg	15 (n = 1)	13.45	0.63	1.00	100.00	0.40	0.16
	10 (n = 4)	8.57 ± 0.77^a	0.66 ± 0.19^a	1.00 ± 0.00^a	100.00 ± 0.00^a	0.29 ± 0.038^a	0.15 ± 0.020^a
	5 (n = 1)	4.58	0.10	1.00	100.00	0.20	0.094

^aMean ± SEM.

^bLaser-power density measured at the tip of objective.

^cEstimated power density by assuming the scattering length at 1030 nm as 175 μm, which is derived from simulations based on experimental data (Papagiakoumou et al., 2013).