TABLE 1.

Eicosanoid metabolites altered with HFD in the kidney

Name	Acronym	MECH	STD	HFD	P	q Value
Up with HFD
AA metabolites
Arachidonic acid	AA	PUFA	243.4 ± 19.2	312.7 ± 40.9	0.010	0.037
13,14-Dihydro-15-keto-prostaglandin D2	dhk-PGD2	COX	0.04 ± 0.01	1.20 ± 0.02	0.008	0.035
5,6-Epoxyeicosatrienoic acid	5,6-EET	P450	1.59 ± 0.74	8.64 ± 5.64	0.014	0.045
11,12-Epoxyeicosatrienoic acid	11,12-EET	P450	0.02 ± 0.01	0.18 ± 0.13	0.015	0.045
5-Oxo-eicosatetraenoic acid	5-oxoETE	LOX	0.22 ± 0.11	1.56 ± 1.21	0.013	0.043
6(R),15(R)-lipoxin A4	6(R),15(R)-LXA4	LOX	0.02 ± 0.01	0.05 ± 0.02	0.006	0.032
6(S)-lipoxin A4	6SLXA4	LOX	0.02 ± 0.01	0.05 ± 0.02	0.015	0.045
9-Hydroxy-eicosatetraenoic acid	9-HETE	NE	0.02 ± 0.01	0.14 ± 0.09	0.013	0.043
DHA metabolites
16,17-Epoxy-docosapentaenoic acid	16,17-EpDPE	P450	0.42 ± 0.07	0.65 ± 0.08	0.001	0.010
16-Hydroxy-docosahexaenoic acid	16-HDoHE	P450	0.60 ± 0.07	1.20 ± 0.24	<0.001	0.003
19,20-Epoxy-docosapentaenoic acid	19,20-EpDPE	P450	1.03 ± 0.19	1.63 ± 0.22	0.001	0.010
19,20-Dihydroxy-docosapentaenoic acid	19,20-DiHDPA	P450	0.55 ± 0.10	1.32 ± 0.43	0.001	0.008
4-Hydroxy-docosahexaenoic acid	4-HDoHE	LOX	1.22 ± 0.20	3.17 ± 1.36	0.007	0.032
7-Hydroxy-docosahexaenoic acid	7-HDoHE	LOX	0.17 ± 0.02	0.32 ± 0.09	0.004	0.021
11-Hydroxy-docosahexaenoic acid	11-HDoHE	LOX	0.25 ± 0.03	0.45 ± 0.06	<0.001	0.001
17-Hydroxy-docosahexaenoic acid	17-HDoHE	LOX	1.04 ± 0.13	1.84 ± 0.31	<0.001	0.005
Protectin DX	PDX	LOX	0.02 ± 0.01	0.07 ± 0.02	0.009	0.037
15(t)-Protectin D1	15(t)-PD1	LOX	4.14 ± 0.89	12.68 ± 3.76	0.001	0.008
8-Hydroxy-docosahexaenoic acid	8-HDoHE	NE	0.67 ± 0.08	1.16 ± 0.24	0.001	0.009
10-Hydroxy-docosahexaenoic acid	10-HDoHE	NE	0.26 ± 0.03	0.45 ± 0.07	0.001	0.007
13-Hydroxy-docosahexaenoic acid	13-HDoHE	NE	0.51 ± 0.10	0.78 ± 0.11	0.003	0.017
20-Hydroxy-docosahexaenoic acid	20-HDoHE	NE	1.61 ± 0.22	2.46 ± 0.40	0.002	0.013
EPA metabolites
11-Hydroxy-eicosapentaenoic acid	11-HEPE	NE	0.28 ± 0.10	2.0 ± 1.50	0.010	0.037
Down with HFD
AA metabolites
Prostaglandin E2	PGE2	COX	2.88 ± 1.02	1.43 ± 0.21	0.011	0.040
Dihomo-γ-LA metabolites
15-Keto-prostaglandin F1α	15-keto-PGF1α	COX	0.43 ± 0.09	0.23 ± 0.08	0.007	0.032
15-Hydroxy-eicosatrienoic acid	15-HETrE	LOX	0.46 ± 0.07	0.34 ± 0.04	0.009	0.037
EPA metabolites
5-Hydroxy-eicosapentaenoic acid	5-HEPE	LOX	0.24 ± 0.03	0.09 ± 0.02	<0.001	0.001
12-Hydroxy-eicosapentaenoic acid	12-HEPE	LOX	4.23 ± 0.31	1.17 ± 0.26	<0.001	<0.001
15-Hydroxy-eicosapentaenoic acid	15-HEPE	LOX	0.15 ± 0.02	0.05 ± 0.01	<0.001	<0.001
18-Hydroxy-eicosapentaenoic acid	18-HEPE	NE	0.22 ± 0.03	0.07 ± 0.01	<0.001	<0.001

All values are picomoles per milligram of renal tissue expressed as mean ± SD. MECH, mechanism.