Fig. 3.

Adoptive transfer of Tregs prevents hepatic lipid synthesis in AFL in mice. C57BL/6 mice were fed a 5% alcohol-containing Leiber-DeCarli diet or isocaloric pair-fed diet for 6 weeks, and livers were subjected to analysis of SREBP-1C mRNA (A) (*P < 0.05, **P < 0.01; n = 8) by real-time PCR. B: Nuclear SREBP-1 levels by Western blotting are shown in representative gels (upper panel) and graphs of density units (lower panel) (*P < 0.05, **P <0.01; n = 6). C: SREBP-1C target gene expression by real-time PCR for ACC1 mRNA and FAS mRNA (*P < 0.05; n = 8). D: The pAMPK(Thr172) and AMPK levels by Western blotting are shown in representative gels (upper panel) and graphs of density units (lower panel) (*P < 0.05, **P < 0.01; n = 6). The values shown in the graphs are mean ± SEM. EtOH, ethanol.