Fig. 4.

Adoptive transfer of Tregs improves the hepatic fatty acid β-oxidation in AFL in mice. C57BL/6 mice were fed a 5% alcohol-containing Leiber-DeCarli diet or isocaloric pair-fed diet for 6 weeks, and livers were subjected to analysis of PPARα mRNA (A) (*P < 0.05, **P < 0.01; n = 8) by real-time PCR. B: Nuclear PPARα levels by Western blotting are shown in representative gels (upper panel) and graphs of density units (lower panel). (*P < 0.05; n = 6). C: Representative sections of immunohistochemistry stained PPARα in livers with brown staining in hepatic nuclei as positive (magnification, ×400). D: PPARα target gene expression by real-time PCR for MCAD mRNA, LCAD mRNA, ACOX mRNA, and CPT1 mRNA (*P < 0.05; n = 8). The values shown in the graphs are mean ± SEM. EtOH, ethanol; ns, nonsignificant.