TABLE 1.
Primer sequences for real-time quantitative PCR gene validation
| Gene | Primer Sequences |
| ACC1-F | ATGGGCGGAATGGTCTCTTTC |
| ACC1-R | TGGGGACCTTGTCTTCATCAT |
| SERBPlc-F | TTGTGGAGCTCAAAGACCTG |
| SERBPlc-R | TGCAAGAAGCGGATGTAGTC |
| PPARa-F | AAACATCGAGTGTCGAATATGTGG |
| PPARa-R | AGCCGAATAGTTCGCCGAAAG |
| CD68-F | CCCACAGGCAGCACAGTGGAC |
| CD68-R | TCCACAGCAGAAGCTTTGGCCC |
| MCP-1-F | TGTGCTGACCCCAAGAAGGA |
| MCP-l-R | TGAGGTGGTTGTGGAAAAGG |
| βactin-F | TCTTTTCCAGCCTTCCTTCTTG |
| βactin-R | GAGGTCTTTACGGATGTCAACG |
| Fas-F | GTGATAGCCGGTATGTCGGG |
| Fas-R | TAGAGCCCAGCCTTCCATCT |
| SREBP1-F | TCTGGAGACATCGCAAACAAG |
| SREBP1-R | TGGTAGACAACAGCCGCATC |
| Cpt1a-F | AGCGACTCTTCAATACTTCCCG |
| Cpt1a-R | CAAAATAGGTCTGCCGACACTTC |
| Acox-F | TCAGATGTCACAGGTAAACAGTCG |
| Acox-R | GTCTTGTTCAGTGGGGACTTCTT |
| LCAD-F | TACAAGGAGTAAGAACGAACGCC |
| LCAD-R | CGACGATCACGAGATCACTTAAC |
| MCAD-F | TAAGACCAAAGCAGAGAAGAAGG |
| MCAD-R | AACACATTTTCCTTAGGCACTCT |
| Acox, acyl-coA oxidase; CPT1, Carnitinepalmitoyltransferase1; Fas, fatty acid synthase; F, forward; R, reverse. | |