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. 2019 Mar 19;60(5):995–1004. doi: 10.1194/jlr.M092494

Fig. 2.

Fig. 2.

The Laurdan dye method confirms that AdipoR1 and AdipoR2 are required to maintain membrane fluidity in HEK293 cells. A: Pseudocolor images showing the Laurdan dye GP index at each pixel position in HEK293 cells challenged with 200 μM PA and treated with NT, AdipoR1, and/or AdipoR2 siRNA. Note the pronounced rigidification of the plasma membrane in the AdipoR1/2 siRNA-treated cells. B: Average GP index from several images as in panel A (n = 10–15). C: Distribution of the GP index values in representative images for each treatment. Error bars show the SDs. ***P < 0.001 compared with the control treatment. ###P < 0.001 compared with the AdipoR1/2 siRNA treatment. GP, generalized polarization; NT, nontarget.