Fig. 2.

LPAR4 deficiency in male mice reduces neutral lipid and CD68⁺ cell content but not α-smooth muscle actin in aortic root atherosclerotic lesions. A: Quantification of lipid content (μm²) from Oil Red O staining of aortas from individual WT (dark circles) or Lpar4^Y/− (open circles) mice (P = 0.001) and representative Oil Red O staining of neutral lipid in aortic root sections (bottom). B: Quantification of CD68⁺ staining from individual WT (dark circles) or Lpar4^Y/− (open circles) mice (P = 0.045) and representative CD68⁺ staining (bottom). C: Quantification of α-smooth muscle actin staining from individual WT (dark circles) or Lpar4^Y/− (open circles) mice (P = 0.27) and representative α-smooth cell actin staining (bottom). In all cases, the area values for each mouse are the average of six measurements taken 80 μM apart.