Fig. 4.

LPAR4 deficiency influences ox-LDL-induced gene expression in aortic SMCs. A: Gene expression analysis from SMCs isolated from WT (gray hatched bars) or Lpar4^Y/− (open bars) aortas. Cells were exposed to ox-LDL (50 μg/ml; green bars) or vehicle control for 3 h. Results are presented relative to expression in WT vehicle-treated cells and are summarized from three separate experiments analyzed by two-way ANOVA with the Mann-Whitney test. E = significant difference in gene expression between environmental exposures (P < 0.05). G = significant difference in gene expression between genotypes (P < 0.05). GE = significant difference in gene expression based on genotype and environmental exposure (P < 0.05). B: Quantification of MYH11 H3K4dime PLA⁺ cells from individual WT (dark circles) or Lpar4^Y/− (open circles) mice. Individual values represent the average of six measurements taken 80 μM apart (P = 0.045). C: Representative immunostaining of aortic root sections from WT (top) and Lpar4^Y/− (bottom) mice with DAPI (blue), ACTA2 (green), and MYH11 H3K4dime PLA signal (red). Scale bar = 50 μm.