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Chromatographic and spectral analysis of SMT-generated C28- and C29-sterol products. A–D: GC profile of total sterol from 24-AcSMT or 28-AcSMT incubated with CHT or ERGT. E: Mass spectrum of compound 22 prepared as indicated for panels E-1 to E-4. Panel E-5 is the UV spectrum of 22, while panels E-6 and E-7 are the NMR with assignment of 22. F, G: GC profiles for total sterol generated from mutant 28-AcSMT assayed with SAM and ERGT.
