Table 1.

Primers for qRT‐PCR

Gene name	Sense primer	Antisense primer	Tm (°C)	GenBank accession number
GAPDH	AGGTCGGTGTGAACGGATTTG	TGTAGACCATGTAGTTGAGGTCA	58	NM_008084.2
SSEA‐1	ACGGATAAGGCGCTGGTACTA	GGAAGCCATAGGGCACGAA	59	NM_010242.3|
PRDM1	TGGAGGACGCTGATATGACT	CTTACCACGCCAATAACCTC	59	NM_007548.3
STELLA	GACCCAATGAAGGACCCTGAA	GCTTGACACCGGGGTTTAG	59	NM_139218.1
STRA8	AGCAGCTTAGAGGAGGTCAAGA	TACTCGGAACCTCACTTTTGTC	57	XM_006505830.1
SCP3	CTAGAATTGTTCAGAGCCAGAGA	GTTCAAGTTCTTTCTTCAAAG	57	NM_011517.2
C‐KIT	TGACTTACGACAGGCTCGTG	AAGGAGTGAACAGGGTGTGG	57	NM_001122733
PRDM14	ACAGCCAAGCAATTTGCACTAC	TTACCTGGCATTTTCATTGCTC	57	NM_001081209.2
SOX2	GCCCAGGAGAACCCCAAGAT	GGGTGCCCTGCTGCGAGTA	58	NM_011443.3
DMRT1	TGGGTTCTGGAAGCAAGAAG	CTGTCTTCTCAGGGCCACCT	58	NM_015826.5
GFRa1	GACCGTCTGGACTGTGTGAAAG	TTAGTGTGCGGTACTTGGTGC	58	XM_006526685.1
VASA	TATGTGCCTCCCAGCTTCAGTA	CTGGATTGGGAGCTTGTGAAGA	59	XM_006517526.1
AP2r	TGAAGATGAAGCTGGGCTTT	TCCATTCTCTTCCGGTTCAG	58.5	NM_009335.2
NANOG	ATGAAGTGCAAGCGGTGGCAGAAA	CCTGGTGGAGTCACAGAGTAGTTC	59	NM_028016.2