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Primer sequences of target genes and GAPDH for real‐time PCR assay
| Target gene (mouse) | Primer sequence (5′–3′) |
|---|---|
| Runx2 | F: CCGAACTGGTCCGCACCGAC |
| R: CTTGAAGGCCACGGGCAGGG | |
| OPN | F: GTGGTGATCTAGTGGTGCCAAGAGT |
| R: AGGCACCGGCCATGTGGCTAT | |
| OCN | F: GTCCTATGGCGGGGAGGACTGG |
| R: TGGCAGCTGCAAGCTCTCTGTA | |
| GAPDH | F: TATGACTCTACCCACGGCAAGT |
| R: ATACTCAGCACCAGCATCACC |
