Figure 2.

Up‐regulation of Anxa2 increased proliferation of MCF ‐7 cells. (a) Living cells were counted using a cytometer every day for 6 consecutive days. Transfectants with Anxa2 overexpression proliferated much faster than control cells. *P < 0.05 versus control. (b) Colony formation was significantly higher in Anxa2‐overexpressing transfectants of MCF‐7 cells compared to wild‐type and control cells. Bar charts show average number of colonies and comparison between MCF‐7/Control cells and MCF‐7/Anxa2 groups. Statistical significance was assessed by one‐way ANOVA, **P < 0.01 versus control. (c): Result of Beckman Coulter EPICS analyzer showed that up‐regulation of Anxa2 induced increase in proportion of G₂/M + S phase cells compared to controls. Clone1–3: Transfectants stably overexpressing Anxa2. Data are mean ± SD. Experiments were repeated at least three times.