Table II. Eighteen putative extracellular KLK7 substrates identified in the TAILS analysis of SKOV-3 cell conditioned media.
| Accession | Symbol | Protein Description | P1 | Peptides | m/z | Precursor charge | Probability |
|---|---|---|---|---|---|---|---|
| Peptidases and peptidase regulators | |||||||
| #P03956 | MMP1 | Matrix metallopeptidase 1 | 70 | F.FGLKVTGKPDAETLKVMKQPR.C | 838.5505 | 3 | 1 |
| 71 | F.GLKVTGKPDAETLKVMKQPR.C | 789.5278 | 3 | 1 | |||
| 116 | Y.RIENYTPDLPR.A | 469.9315 | 3 | 0.9889 | |||
| 149 | F.SGDVQLAQDDIDGIQAIYGR.S | 723.3728 | 3 | 1 | |||
| *#P08253 | MMP2 | Matrix metallopeptidase 2 | 80 | F.FGLPQTGDLDQNTIETMR.K | 1035.5226 | 2 | 1 |
| #P09238 | MMP10 | Matrix metallopeptidase 10 | 99 | F.SSFPGMPKWR.K | 420.9112 | 3 | 1 |
| Pathway inhibitors | |||||||
| #Q9UBP4 | DKK3 | Dickkopf WNT signaling pathway inhibitor 3 | 128 | F.SETVITSVGDEEGRR.S | 834.9341 | 2 | 0.9987 |
| ECM/basement membrane constituents and ECM/growth factor regulators | |||||||
| #Q02388 | COL7A1 | Collagen, type VII, alpha 1 | 2819 | Y.AADTAGSQLHAVPVLR.V | 547.3162 | 3 | 0.9991 |
| #Q99715 | COL12A1 | Collagen, type XII, alpha 1 | 1139 | D.NTVVLEELR.A | 553.8351 | 2 | 0.9452 |
| 1138 | Y.DNTVVLEELR.A | 611.3486 | 2 | 0.9986 | |||
| 2926 | M.LNQIPNDYQSSR.N | 734.8836 | 2 | 0.9924 | |||
| #P35555 | FBN1 | Fibrillin 1 | 1760 | Y.TGLPVDIDECR.E | 654.8376 | 2 | 0.9998 |
| #O95633 | FSTL3 | Follistatin-like 3 | 84 | L.GFLGLVHCLPCKDSCDGVECGPGKACR.M | 1051.1868 | 3 | 1 |
| *P17936 | IGFBP3 | Insulin-like growth factor binding protein 3 | 186 | Y.KVDYESQSTDTQNFSSESKR.E | 813.4225 | 3 | 1 |
| P24592 | IGFBP6 | Insulin-like growth factor binding protein 6 | 165 | R.HLDSVLQQLQTEVYR.G | 932.0126 | 2 | 1 |
| 179 | Y.RGAQTLYVPNCDHR.G | 860.9416 | 2 | 0.8584 | |||
| #Q14112 | NID2 | Nidogen 2 (osteonidogen) | 80 | N.GIISTQDFPR.E | 584.3326 | 2 | 0.8835 |
| 124 | Y.REDTSPAVLGLAAR.Y | 745.4311 | 2 | 0.9988 | |||
| #P09486 | SPARC | Secreted protein, acidic, cysteine-rich (osteonectin) | 179 | Y.ERDEDNNLLTEKQKLR.V | 1052.1161 | 2 | 0.918 |
| #P07996 | THBS1 | Thrombospondin 1 | 258 | Y.IGHKTKDLQAICGISCDELSSMVLELR.G | 1059.2505 | 3 | 1 |
| 665 | Y.LGHYSDPMYR.C | 636.8161 | 2 | 0.9809 | |||
| Cell adhesion molecules/regulators and signal transducers | |||||||
| #P55285 | CDH6 | Cadherin 6, type 2, K-cadherin | 343 | Y.TLKVEASNPYVEPR.F | 835.9911 | 2 | 0.9999 |
| Cytoskeletal proteins/regulators | |||||||
| #P15311 | EZR | Ezrin | 424 | Y.TAKIALLEEAR.R | 641.9221 | 2 | 0.9972 |
| Other (Ion channel, galactoside/carbohydrate binding and immunomodulatory proteins) | |||||||
| #O94985 | CLSTN1 | Calsyntenin 1 | 169 | Y.KATVIEGKQYDSILR.V | 912.0781 | 2 | 0.9999 |
| #Q08380 | LGALS3BP | Lectin, galactoside-binding, soluble, 3 binding protein | 442 | Y.SSDYFQAPSDYR.Y | 735.3416 | 2 | 0.9975 |
| #P07602 | PSAP | Prosaposin | 171 | F.MANIPLLLYPQDGPR.S | 866.4871 | 2 | 0.9998 |
Accession, reviewed UniProt KB accession; Symbol, UniProtKB/Swiss-Prot entry name prefix (all_HUMAN) with HUGO gene name for UniProtKB entries; Protein Description, UniProtKB protein description; P1, KLK7 cleaves C-terminal to; Peptides, TAILS-identified peptide cleavage sites with the “.” representing where the cleavage is identified; *Established KLK7 substrates.