Table IV. Thirty-seven putative extracellular KLK7 substrates identified in the TAILS analysis of OVMZ-6 cell conditioned media.
| Accession | Symbol | Protein Description | P1 | Peptides | m/z | Precursor charge | Probability |
|---|---|---|---|---|---|---|---|
| Peptidases and peptidase regulators | |||||||
| #P01034 | CST3 | cystatin C | 125 | F.QIYAVPWQGTMTLSKSTCQD.A | 1227.1276 | 2 | 0.9996 |
| 128 | Y.AVPWQGTMTLSKSTCQD.A | 1025.0246 | 2 | 1 | |||
| ¥Q92743 | HTRA1 | HtrA serine peptidase 1 | 286 | F.SLQNTVTTGIVSTTQR.G | 870.4891 | 2 | 1 |
| P06744 | GPI | glucose-6-phosphate isomerase | 426 | L.ANFLAQTEALMR.G | 699.8846 | 2 | 0.9971 |
| Q9Y287 | ITM2B | integral membrane protein 2B | 254 | F.ENKFAVETLIC.S | 739.9136 | 2 | 0.9999 |
| P10646 | TFPI | tissue factor pathway inhibitor | 247 | Y.SGCGGNENNFTSKQECLR.A | 1063.5051 | 2 | 1 |
| 209 | F.EFHGPSWCLTPADR.G | 853.9121 | 2 | 1 | |||
| Pathway inhibitors | |||||||
| #Q9UBP4 | DKK3 | dickkopf WNT signaling pathway inhibitor 3 | 128 | F.SETVITSVGDEEGRR.S | 834.9341 | 2 | 0.9998 |
| ECM/basement membrane constituents and ECM/growth factor regulators | |||||||
| ¥Q14512 | FGFBP1 | fibroblast growth factor binding protein 1 | 97 | A.GNPTSCLKLKDER.V | 810.4851 | 2 | 0.9996 |
| P51858 | HDGF | hepatoma-derived growth factor | 79 | R.KGFSEGLWEIENNPTVKASGY.Q | 810.1128 | 3 | 0.9991 |
| #P24592 | IGFBP6 | insulin like growth factor binding protein 6 | 219 | G.KSLPGSPDGNGSSSCPTGSS.G | 1002.4841 | 2 | 0.9999 |
| 218 | M.GKSLPGSPDGNGSSSCPTGSS.G | 1030.9951 | 2 | 1 | |||
| 179 | Y.RGAQTLYVPNCDHR.G | 860.9416 | 2 | 0.9365 | |||
| *P21741 | MDK | midkine (neurite growth-promoting factor 2) | 88 | F.ENWGACDGGTGTKVR.Q | 838.4281 | 2 | 1 |
| 87 | K.FENWGACDGGTGTKVR.Q | 911.9626 | 2 | 1 | |||
| 29 | K.KGGPGSECAEWAWGPCTPSSKDCGVGFR.E | 1048.1725 | 3 | 0.9963 | |||
| 86 | Y.KFENWGACDGGTGTKVR.Q | 993.0416 | 2 | 1 | |||
| #P09486 | SPARC | secreted protein, acidic, cysteine-rich (osteonectin) | 179 | Y.ERDEDNNLLTEKQKLR.V | 1052.1161 | 2 | 0.9983 |
| Cell adhesion molecules/regulators and signal transducers/cell surface receptors | |||||||
| ¥P05067 | APP | amyloid beta precursor protein | 433 | Q.HFQEKVESLEQEAANER.Q | 1056.5531 | 2 | 1 |
| P63092 | GNAS | GNAS complex locus | 318 | Y.TTPEDATPEPGEDPR.V | 823.3916 | 2 | 1 |
| Q13421 | MSLN | mesothelin | 67 | L.GFPCAEVSGLSTER.V | 772.3851 | 2 | 0.9957 |
| O95631 | NTN1 | netrin 1 | 251 | F.SRLHTFGDENEDDSELAR.D | 1063.0036 | 2 | 0.9983 |
| 254 | L.HTFGDENEDDSELAR.D | 884.8951 | 2 | 1 | |||
| Q99985 | SEMA3C | semaphorin 3C | 499 | Y.VSSNEGVSQVSLHR.C | 766.9156 | 2 | 1 |
| Q08629 | SPOCK1 | sparc/osteonectin, cwcv and kazal-like domains proteoglycan (testican) 1 | 22 | R.HLDALAGGAGPNHGN.F | 717.8686 | 2 | 1 |
| Q9UDY2 | TJP2 | tight junction protein 2 | 459 | F.KSTGDIAGTVVPETNKEPR.Y | 1051.1211 | 2 | 0.9998 |
| Cytoskeletal proteins/regulators | |||||||
| ¥P61160 | ACTR2 | ARP2 actin-related protein 2 homolog (yeast) | 356 | L.GGAVLADIMKDKDNFWMTR.Q | 1135.6296 | 2 | 1 |
| #P15311 | EZR | ezrin | 547 | R.THNDIIHNENMR.Q | 764.3811 | 2 | 0.9998 |
| 579 | R.IDEFEA.L | 764.3811 | 2 | 0.9998 | |||
| 295 | R.KPDTIEVQQM.K | 628.8631 | 2 | 0.9973 | |||
| O75781 | PALM | paralemmin | 56 | L.EGTPSSASEGDEDLRR.Q | 870.4161 | 2 | 1 |
| ¥Q9Y490 | TLN1 | talin 1 | 1575 | F.ASNPEFSSIPAQISPEGR.A | 960.9971 | 2 | 1 |
| Other (Ion channel, galactoside/carbohydrate/lipid binding and transporters | |||||||
| #P61769 | B2M | beta-2-microglobulin | 85 | L.YYTEFTPTEKDEYACR.V | 1071.0121 | 2 | 0.9999 |
| 87 | Y.TEFTPTEKDEYACR.V | 907.9486 | 2 | 1 | |||
| 86 | Y.YTEFTPTEKDEYACR.V | 989.4806 | 2 | 1 | |||
| 108 | S.QPKIVKWDRD.M | 759.4716 | 2 | 0.9996 | |||
| P54105 | CLNS1A | chloride channel, nucleotide-sensitive, 1A | 168 | Y.TYEEGLSHLTAEGQATLER.L | 1070.0426 | 2 | 0.9998 |
| #O94985 | CLSTN1 | calsyntenin 1 | 169 | Y.KATVIEGKQYDSILR.V | 912.0781 | 2 | 1 |
| Q6UVK1 | CSPG4 | chondroitin sulfate proteoglycan 4 | 1856 | L.SVVDPDSAPGEIEYEVQR.A | 1012.5051 | 2 | 1 |
| P17931 | LGALS3 | lectin, galactoside-binding, soluble, 3 | 107 | Y.GAPAGPLIVPYNLPLPGGVVPR.M | 1094.6566 | 2 | 1 |
| #Q08380 | LGALS3BP | lectin, galactoside-binding, soluble, 3 binding protein | 442 | Y.SSDYFQAPSDYR.Y | 735.3416 | 2 | 0.9997 |
| P29122 | PCSK6 | proprotein convertase subtilisin/kexin type 6 | 302 | Y.SASWGPDDDGKTVDGPGR.L | 942.9716 | 2 | 1 |
| ¥Q8NBP7 | PCSK9 | proprotein convertase subtilisin/kexin type 9 | 418 | F.SAKDVINEAWFPEDQR.V | 987.0236 | 2 | 1 |
| 400 | L.SAEPELTLAELR.Q | 681.8881 | 2 | 0.9976 | |||
| 183 | Y.LLDTSIQSDHREIEGR.V | 952.0081 | 2 | 0.9919 | |||
| #P07602 | PSAP | prosaposin | 171 | F.MANIPLLLYPQDGPR.S | 866.4871 | 2 | 0.9995 |
| 172 | M.ANIPLLLYPQDGPR.S | 800.9671 | 2 | 1 | |||
| O95810 | SDPR | serum deprivation response | 395 | Y.EGSYALTSEEAER.S | 738.3571 | 2 | 0.9998 |
| Q9ULF5 | SLC39A10 | solute carrier family 39 (zinc transporter), member 10 | 80 | F.GLEKLLTNLGLGER.K | 791.0041 | 2 | 1 |
| O14745 | SLC9A3R1 | solute carrier family 9, subfamily A (NHE3, cation proton antiporter 3), member 3 regulator 1 | 180 | R.SVDPDSPAEASGLR.A | 717.8676 | 2 | 0.9942 |
| Oxidative stress-related | |||||||
| O43827 | ANGPTL7 | angiopoietin like 7 | 271 | F.STKDKDNDNCLDKCAQLR.K | 773.0898 | 3 | 0.9987 |
| Inflammatory response | |||||||
| ¥P04083 | ANXA1 | annexin A1 | 56 | M.VKGVDEATIIDILTKR.N | 625.0787 | 3 | 0.9983 |
| O95407 | TNFRSF6B | tumor necrosis factor receptor superfamily member 6b | 233 | L.QALEAPEGWGPTPR.A | 771.9096 | 2 | 0.9891 |
| 261 | L.TELLGAQDGALLVR.L | 745.4436 | 2 | 0.9998 | |||
| 90 | Y.CNVLCGEREEEAR.A | 828.3881 | 2 | 0.944 |
Accession, reviewed UniProt KB accession; Symbol, UniProtKB/Swiss-Prot entry name prefix (all _HUMAN) with HUGO gene name for UniProtKB entries; Protein Description, UniProtKB protein description; P1, KLK7 cleaves C-terminal to; Peptides, TAILS-identified peptides with “.” indicating respective semi-tryptic cleavage sites; *Established KLK7 substrate; #Substrates identified in both SKOV-3 and OVMZ-6 cell CM by TAILS analysis; ¥Substrates identified in qPROTOMAP and TAILS analysis of SKOV-3 and OVMZ-6 cell CM respectively.