Fig. 3.
Proteins related to alternative carbon sources and respiration. Assignment of proteins to pathogen (S. aureus) and host (HBE) is indicated on top of the respective protein groups. Because of limited carbon sources post infection, alternative pathways for energy consumption are upregulated. Substrates like glycerol and fatty acids are being consumed during intracellular conditions. Another major adjustment is related to oxygen availability which affects the pathways related to fermentation and oxidative phosphorylation. The protein quantities were derived from the mass spectrometry measurements of four biological replicates. Time trends with significant changes (p value < 0.01) were fitted into different clusters depending on their behavior (supplemental Fig. S2). The assigned cluster is presented in the column “C.” The color coding is based on the standard deviation of each set of data. σS. aureus = 2.34 and σHBE = 0.33. HIF: Hypoxia-Inducible Factor.