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Direct assessment of sPLA2s activities of by mass spectrometry. A, Control experiment with only DOPC. B, DOPC incubated with A. mellifera PLA2. C, DOPC incubated with Cdpi-P1 fraction D, DOPC incubated with C. purpurascens I.V. Notice that compared with the control, PLA2s hydrolyzes the DOPC (786 Da) to form the lysophospholipid (522 Da). Sodium and potassium adducts could be observed.
