Fig. 4.

Modulation of the 19S-20S interaction by oxidative stress. A, Plot depicting normalized SILAC ratios (i.e. log₂ L/H) of eight unique linkages (1–8) bridging the 19S and 20S subcomplexes with significantly decreased abundance in H₂O₂ treated samples (log₂ (L/H) ≤ 1). B, These linkages were mapped to the high-resolution structure of the human 26S proteasome (PDB: 5GJR). Inset shows a cut-away of the rotated 26S complex depicting the distribution of intersubcomplex cross-links along the opposing faces of the ATPase ring and 20S α-ring.