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. 2015 Jul 31;48(5):550–560. doi: 10.1111/cpr.12200

Figure 1.

Figure 1

FGF13 affected C2C12 cell proliferation. (a) mRNA expression levels of FGF13, MyOG and MyHC during C2C12 myoblast differentiation. (b) C2C12 cells were transfected with empty vector during proliferation as a control group. (c) C2C12 cells were transfected with FGF13 overexpression plasmids during proliferation. The cells were harvested, and samples were analysed by flow cytometry at 72 h post‐transfection. (d) percentages of cells in G1 and S phases were analysed statistically in (b) and (c). (e) FGF13 was overexpressed in C2C12 cells during proliferation, and cyclin E and cyclin B1 protein levels were analysed by western blotting after 72 h. Results are presented as the mean ± SEM. n = 3. *P < 0.05.