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. 2001 Dec 11;98(26):14883–14888. doi: 10.1073/pnas.261477898

Figure 1.

Figure 1

Plasmid templates for analysis of the mechanism of glnAp2 promoter activation by NtrC-dependent enhancer. Strong and weak NtrC-binding sites are indicated by closed and open boxes, respectively. Under our experimental conditions, only the strong sites are occupied and contribute to the enhancer activity (11). The pTH8 and pLR100 plasmids (3.6 and 3.3 kb in size, respectively) have 110-bp wild type (wt) enhancer–promoter spacing. The pLY10, pLY11, and pLY12 plasmids (7.6, 7.4, and 7.4 kb in size, respectively) have 2.5 kb, 2.5 kb, and 2.3 kb enhancer–promoter distances (the enhancer–promoter distances on the other side of the enhancer are indicated in italics). pAN6 has no strong NtrC-binding sites. The transcripts were terminated at the T7 terminator positioned at different distances downstream of the promoter. The lengths of the transcripts are indicated.