TABLE 1.
Top hits obtained from LC-MS/MS analysis of cyst wall fractionsa
| ToxoDB ID
(TgME49_) |
Protein | WT/CST1-KO | Bradyzoite
percentile |
Other studies
(PMID) |
|---|---|---|---|---|
| 264660 | CST1 | 4,885 | 98.2 | 24385904 |
| 253330 | BPK1 | 134 | 99.5 | 23291621 |
| 203290 | GRA34 | 111 | 98.8 | 27486190 |
| 251540 | GRA9 | 82 | 97.1 | 15491588 |
| 270240 | MAG1 | 74 | 99.3 | 7808478 |
| 209755 | Hypothetical protein | 73 | 99.0 | NA |
| 208740 | MCP3 | 63 | 98.5 | 19901027 |
| 204340 | Hypothetical protein | 55 | 90.0 | 19218426 |
| 213067 | GRA36 | 52 | 92.3 | 27486190 |
| 258870 | Hypothetical protein | 45 | 93.0 | NA |
| 227280 | GRA3 | 44 | 97.9 | 8195173 |
| 221620 | Beta-tubulin, putative | 38 | 91.0 | 19886702 |
| 239740 | GRA14 | 34 | 94.5 | 18765740 |
| 208730 | MCP4 | 31 | 96.3 | 22021236 |
| 319340 | CST5/GRA49 (this study) | 27 | 97.3 | 21920448 |
| 254720 | GRA8 | 26 | 93.7 | 10613696 |
| 200360 | Hypothetical protein | 25 | 98.8 | 23027733 |
| 286450 | GRA5 | 23 | 99.7 | 8515776 |
| 203600 | CST2/GRA47 (this study) | 22 | 81.4 | 30362762 |
| 222170 | GRA17 | 21 | 93.9 | 25974303 |
| 203310 | GRA7 | 21 | 98.6 | 9566518 |
| 254470 | MYR1 | 20 | 82.2 | 26838724 |
| 260520 | CST6/GRA50 (this study) | 20 | 96.2 | NA |
| 220240 | GRA31 | 19 | 84.2 | 27486190 |
| 226380 | GRA35 | 18 | 95.5 | 27486190 |
| 297880 | GRA23 | 17 | 92.6 | 23583316 |
| 219820 | Polyubiquitin UbC, putative | 17 | 96.4 | NA |
| 270320 | PPM3C | 16 | 92.3 | 28556455 |
| 227620 | GRA2 | 15 | 99.3 | 8384696 |
| 263300 | Porin protein, putative | 15 | 88.2 | NA |
| 230705 | CST3/GRA48 (this study) | 15 | 83.5 | NA |
| 288650 | GRA12 | 13 | 99.0 | 18840447 |
| 247440 | GRA33 | 13 | 94.4 | 27486190 |
| 290700 | GRA25 | 13 | 93.2 | 24711568 |
| 279100 | MAF1-related protein | 12 | 84.5 | 26920761 |
| 310780 | GRA4 | 12 | 95.9 | 1362450 |
| 269690 | GRA29 | 11 | 96.1 | 27486190 |
| 261650 | CST4 (this study) | 11 | 92.1 | NA |
The proteins identified are ranked in order of relative abundance using total spectrum counts in the WT strain and comparing to the total spectrum counts in the CST1-KO strain (WT + 1/CST1-KO + 1). An arbitrary WT/CST1-KO cutoff value of ≥10 was used to report top hits from cyst wall fractions in this table. All proteins identified from LC-MS/MS are provided in Data Set S1 in the supplemental material. Bradyzoite percentile values were obtained from transcriptomic data deposited into ToxoDB, Version 40 (Bradyzoite in vivo transcriptome [M4] [6]). Other studies in which proteins on this list were previously described are referenced by PubMed ID number (PMID). NA, not available.