FIG 4.
In vivo production of mccA monocistronic transcripts. (A) The structure of minimized mcc operon and expected transcripts. The mcc operon promoter Pmcc and transcription terminator located between the mccA and mccB genes are indicated. Ribosomes bound to ORFs are schematically shown. SD, Shine-Dalgarno sequences. Binding of the ribosome to mccA induces transcription termination (12). (B) Northern blot hybridization analysis of short monocistronic mccA mRNA abundance in cells harboring mcc plasmids with different mccA gene lengths. Radiolabeled hybridization probe was complementary to an invariant fragment of the mccA gene.