FIG 5.

In vitro translation efficiency of mccA mRNA. Results of translation reiterative initiation experiment with different mccA mRNAs are shown. In vitro translation reactions in the presence or in the absence of ribosome-sequestering competitor mRNA were performed in parallel. Reaction aliquots were taken at different time points, and the number of MccA peptides synthesized per active ribosome was calculated. Amount of peptide synthesized at each time point without competition (N) was plotted versus amount of peptide synthesized with competition (n) at the same time point. Dotted lines indicate a case when n = N (ribosome never leaves mRNA, n_rec = ∞). Data were fitted according to the model described in reference 13, fitting curves are shown as black lines, and respective n_rec is shown by the colored dashed lines.