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Bioactivity of elongated McC-like compounds. (A) MALDI-TOF MS spectra of in vitro-synthesized microcin species. A7 (MRTGNAN), A11 (MGGGMRTGNAN), and A15 (MASTAGGGMRTGNAN) peptides and their N-terminally formylated variants (marked with the f prefix) were adenylated and aminopropylated in vitro. ap, aminopropyl. (B) MICs of indicated microcin species measured by the spot test on the lawns of McC-sensitive E. coli BL21(DE3) cells.
