FIG 3.

Dihydroartemisinin (DHA) survival of k13-mutant, pfcrt-modified lines. Tightly synchronized parasites at the early ring (0 to 3 hpi) (A) or mid-trophozoite (28 to 31 hpi) (B) developmental stage were exposed to a 3-h pulse of DHA at the indicated concentrations. Parasite viability was determined by flow cytometry at 72 hpi. Bar graphs correspond to the mean plus SEM percent survival, equivalent to the parasitemia of DHA-treated parasites divided by the parasitemia of vehicle control (DMSO)-treated parasites. Data are from three or four independent experiments, conducted in duplicate. Statistical significance was determined by two-tailed Student’s t tests (Graphpad Prism 7). *, P < 0.05; **, P < 0.01; ***, P < 0.001.