Figure 1.

Viability assay of C2C12 cells in the presence of insulin and inhibitors of insulin signalling. Polynomial regression curves third order. Top: MTT assay; bottom: neutral red. Effect of insulin (10 nm, solid triangles), PD98059 (50 µm) being present 30 min prior to insulin (10 nm) addition (closed triangles upside down), LY294002 (20 µm) being present 30 min prior to insulin (10 nm) addition (closed diamonds), or PD98059 (50 µm) and LY294002 (20 µm) both being present 30 min prior to insulin (10 nm) addition (closed circles), Ctrl (DM, solid squares). Each experiment was performed in eight replicates and repeated at least twice with similar results.