Western blot analysis of Akt, Ser473‐phospho‐Akt, and myogenin protein expression in C2C12 muscle cells during five subsequent days of experiment. From top to bottom: effect of insulin (10 nm), PD98059 (50 µm) being present 30 min prior to insulin (10 nm) addition, LY294002 (20 µm) being present 30 min prior to insulin (10 nm) addition, or PD98059 (50 µm) and LY294002 (20 µm) both being present 30 min prior to insulin (10 nm) addition. Each experiment was repeated at least twice with similar results.