Figure 8.

Effect of insulin (1, 10 nm) on the expression of nuclear encoded subunit IV and mitochondrial encoded subunit I of COX or Akt and Ser473‐phospho‐Akt in L6 muscle cells. (a) Western blot analysis of the nuclear encoded subunit IV and mitochondrial encoded subunit I of COX protein expression of mitochondrial COX in L6 muscle cells on the fourth, sixth, eighth and tenth day of experiment. (b) Western blot analysis of Akt and Ser473‐phospho‐Akt in L6 muscle cells on the second, fourth, sixth, eighth and tenth day of experiment. From left to the right: Ctrl, effect of insulin (1 nm) and insulin (10 nm). Each experiment was repeated at least twice with similar results.