Figure 9.

Images from confocal microscopy showing intracellular localization of subunit I (a) or subunit IV (b) of mitochondrial COX in cell cultures of L6 muscle cells on the sixth day of experiment. (a) Insulin induces subunit I protein expression of mitochondrial COX in L6 rat myoblasts. (b) Insulin induces subunit IV protein expression of mitochondrial COX in L6 rat myoblasts. Bottom panel: merged pictures of the same visual fields to show nuclei within the cells. From left: negative control (without primary antiserum, CTRL−), positive control (without insulin, CTRL+), effect of insulin (1 nm), effect of insulin (10 nm), effect of LY294002 (20 µm) being present 30 min prior to insulin (10 nm) addition. Images were gathered as single slides under 40× lens and 5× digital zoom. Typical results from two experiments are shown.